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. Author manuscript; available in PMC: 2010 Oct 9.
Published in final edited form as: Mol Cell. 2010 Apr 9;38(1):17–28. doi: 10.1016/j.molcel.2010.02.029

Fig 3. Knock-down of Nrf1 in WT MEFs abolishes MG132-mediated upregulation of RNA levels of PSM genes.

Fig 3

(A) WT and Nrf1-/- MEFs were transduced with retrovirus expressing sh-Nrf1 or vector control and 72 hrs later treated with MG132 for 10 hrs as indicated. The cell lysates were then used for immunoblotting to analyze protein levels of Nrf1 with either a rabbit polyconal antibody specific for the N-terminus or a mouse polyclonal antibody specific for the C-terminal region of Nrf1. β-actin protein levels were used as loading control. (B) RNA from MEFs under the same viral transduction and treatment conditions as above was used for quantitative RT-PCR to assess the mRNA levels of representative PSM genes. The values were normalized to GAPDH and for each cell line the vector-transduced DMSO-treated sample was set to 1. Error bars denote SD (n=3).