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. Author manuscript; available in PMC: 2010 Oct 9.
Published in final edited form as: Mol Cell. 2010 Apr 9;38(1):17–28. doi: 10.1016/j.molcel.2010.02.029

Fig 4. Forced expression of Nrf1 in Nrf1-/- MEFs reinstates MG132-mediated upregulation of RNA levels of PSM genes.

Fig 4

(A) Nrf1-/- MEFs were transduced with retrovirus expressing one of Flag-Nrf1, Flag-Nrf1-HA or vector control and 72 hrs later treated with MG132 for 10 hrs as indicated. The cell lysates were used for immunoblotting to detect the levels of exogenous Nrf1 by using tag-specific antibodies. β-actin protein levels were used as loading control. (B) RNA from Nrf1-/- MEFs under the same viral transduction and treatment conditions as above was used for quantitative RT-PCR to assess the mRNA levels of representative PSM genes. The values were normalized to GAPDH and the vector-transduced DMSO-treated sample was set to 1. Error bars denote SD (n=3).