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. 2010 Mar 23;59(6):1469–1477. doi: 10.2337/db09-0502

FIG. 2.

FIG. 2.

Allogeneic islet grafts harvested from tolerant animals >150 days post-transplant exhibit minimal immune infiltrate and intact islets. Renal subcapsular islet grafts were harvested from animals surviving >150 days after receiving CTLA4-Ig monotherapy or CTLA4-Ig/EP1013 combination therapy. A: Cryosections of islet grafts were co-stained for insulin (TRITC; red) and either CD4 (FITC, green; left panels) or CD8 (FITC; right panels). DAPI nuclear stain (blue) was used to identify all cells present on the section. Representative slides from both treatment groups are shown at 200× magnification and demonstrate that the transplanted islets were largely intact with no invasive immune infiltrate and rare CD4+ or CD8+ lymphocytes. B: Quantification of CD4 and CD8 staining confirmed that CD4+ and CD8+ lymphocytes were infrequently observed within the sections, regardless of the initial treatment intervention. For these data, n = 10 sections from >5 islet grafts per group were blinded and subsequently analyzed. (A high-quality digital color representation of this fiugre is available in the online issue.)