FIG. 5.

Intrinsic expression of Idd9 genes within the CD8⁺ T-cell does not mediate CD8⁺ tolerance by Idd9 genes. Purified NOD or Idd9 Thy1.1⁺ CD8⁺ T-cells (4 × 10⁶) from 3-week-old donor mice were cotransferred with partially (90%) CD8⁺ T-cell–depleted Thy1.2⁺ NOD spleen and LN cells (30 × 10⁶) into NOD-SCID mice. After 10 weeks, mice were infected with Vac-IGRP and IGRP-tetramer⁺ CD8⁺ T-cells measured in the spleen 7 days later. A: Frequency of IGRP-specific cells among all CD8⁺ T-cells; pooled data from two experiments are shown and indicated by circles and triangles; horizontal lines depict mean values. B: Thy1.1 and IGRP-specific tetramer staining of CD8⁺ T-cells; one representative animal shown.