FIG. 7.

CD4⁺ T-cells expressing Idd9 genes are sufficient to restore CD8⁺ tolerance in NOD mice. Purified NOD or Idd9 Thy1.2/CD45.1 CD4⁺ T-cells (6 × 10⁶) were cotransferred with 1 × 10⁷ NOD-Thy1.1 or NOD-CD45.2 congenic CD4⁺-depleted spleen and LN cells, all from 4-week-old donor mice, into NOD-SCID mice. After 10 weeks, mice were infected with Vac-IGRP and IGRP-tetramer⁺ CD8⁺ T-cells measured in the spleen 7 days later. Pooled data from three experiments are shown; NOD (5.6%, IQR 2.7–13%) vs. Idd9 (1%. IQR 0.7–2.1%) P = 0.027 (*). In the second (circles) and third (squares) experiments the CD4⁺ T-cells were purified by FACS sorting. Horizontal line depicts median value.