Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Characteristicsa | Source or reference |
|---|---|---|
| Strains | ||
| P. putida S12 | Wild-type, ATCC 700801 | (Hartmans et al. 1990) |
| P. putida S12_427b | P. putida S12 with an enhanced tyrosine production | (Wierckx et al. 2008) |
| P. putida S12_427 pJTTpal | P. putida S12 427 containing plasmid pJTTpal | This study |
| P. putida S12palB1 | pobA knockout strain derived from P. putida S12 427 containing plasmid pJTTpal | (Verhoef et al. 2007) |
| P. putida S12B2 | pobA and hpd knockout strain derived from P. putida S12 427 | This study |
| P. putida S12palB2 | P. putida S12B2 containing plasmid pJT’Tpal | This study |
| P. putida S12pal_mfsB2 | P. putida S12B2 containing plasmids pJT’Tpal and pBNTmfs | This study |
| P. putida S12B3 | Multidrug efflux MFS transporter (PP1271-PP1273) knockout strain derived from P. putida S12B2 | This study |
| P. putida S12palB3 | P. putida S12B3 containing plasmid pJT’Tpal | This study |
| P. putida S12pal_mfsB3 | P. putida S12palB3 containing plasmids pJT’Tpal and pBNTmfs | This study |
| P. putida S12 pJTTpal | P. putida S12 containing plasmid pJTTpal | (Nijkamp et al. 2005) |
| P. putida S12 pJNNmcs(t) | P. putida S12 containing plasmid pJNNmcs(t) | (Wierckx et al. 2005) |
| Escherichia coli DH5α | supE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | (Sambrook et al. 1982) |
| Plasmids | ||
| pJTTpalc | Apr Gmr, expression vector containing the pal gene under control of the tac promoter (pALter-EX1, U47102) and the tac RBS | (Nijkamp et al. 2007) |
| pJT’Tmcs | Apr Gmr, vector for Ptac and tac RBS controlled expression | This study |
| pJT’Tpal | Apr Gmr, Pal/Tal expression vector under control of Ptac and tac RBS | This study |
| pJNNmcs(t) | Apr Gmr, vector for PnagAa and nagAa RBS controlled expression | (Wierckx et al. 2005) |
| pBNTmcs(Gmr) | Gmr, vector for PnagAa and tac RBS controlled expression | This study |
| pBNTmfs(Gmr) | Gmr, multidrug efflux MFS transporter (PP1271-PP1273) expression vector under control of PnagAa and tac RBS | This study |
| pJQ200SK | Gmr, Suicide vector, P15A ori sacB RP4 pBluescriptSK MCS | (Quandt and Hynes 1993) |
| pJQpobA::tetAloxP | Gmr Tcr, pJQ200SK containing the loxP-tetA-loxP interrupted pobA gene | This study |
| pJQhpd::tetAloxP | Gmr Tcr, pJQ200SK containing the loxP-tetA-loxP interrupted hpd gene | This study |
| pJQmfs::tetAloxP | Gmr Tcr, pJQ200SK containing the loxP-tetA-loxP interrupted multidrug efflux MFS transporter (PP1271-PP1273) | This study |
aApr, Gmr and Tcr, ampicillin, gentamicin and tetracycline resistance, respectively
bPreviously known as P. putida S12 TPL3c
cPreviously known as pTacpal