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. Author manuscript; available in PMC: 2010 May 23.
Published in final edited form as: J Mass Spectrom. 2008 Apr;43(4):518–527. doi: 10.1002/jms.1342

Fig. 3.

Fig. 3

a) Representative spectra for comparison of analysis at ~30 pmol/spot vs. ~1 pmol/spot for peptide 4 (RRLIEDNE(p)YTARG) at phosphopeptide inputs of 40% and 78% spotted with CHCA matrix. b) Variation in the observed phosphopeptide signals at the different concentrations falls within typical variance of the measurements: 30 pmol = black open circles; 1 pmol = grey hatches.