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. Author manuscript; available in PMC: 2011 Feb 15.
Published in final edited form as: Cancer Res. 2010 Feb 2;70(4):1334–1343. doi: 10.1158/0008-5472.CAN-09-2795

Figure 3.

Figure 3

Confirmation of engraftment 4 wk after transplant. LacZ+ Mekk3Δflox/+ or LacZ+ Mekk3Δflox/− BM cells were injected into lethally irradiated nude mice. To confirm engraftment, BM cells from the Mekk3Δflox/+ and Mekk3Δflox/− BM–transplanted mice were obtained 4 wk following transplantation and analyzed by Western blot (A) and genotyping (B and C). B, PCR primers P1, P2, and P3 were used to detect the floxed Mekk3 allele (flox, 1,000 bp), wild-type Mekk3 allele (wt, 900 bp), and Mekk3 germline knockout allele (KO, 830 bp). C, primers P3 and P4 were used to detect wild-type (1,300 pb), floxed (1,500 bp), and the loxp-deleted Mekk3 allele (Δflox, 650 bp). Primer design necessitates using two separate PCR reactions to confirm the presence of the Mekk3 Δflox allele, which represents the deleted Mekk3 allele following tamoxifen treatment. Floxed allele is equivalent to the wild-type allele and the Δflox allele is equivalent to the knockout allele.