Table 1.

Expression levels of natural and recombinant bomapin in cells

Cell line	Bomapin (ng bomapin/mg total protein)
HL-60	0.55 ± 0.02
U937	1.85 ± 0.29
THP-1	2.44 ± 0.58
K562 expressing bomapin-EGFP	1.29 ± 0.26
K562 expressing C395S bomapin-EGFP	1.23 ± 0.21
HT1080 expressing bomapin-EGFP	2.58 ± 0.32

Exponentially growing cells were lysed in a lysis buffer containing protease inhibitor cocktail, and bomapin level was quantified by bomapin-specific ELISA (as described in Methods section).