Table 5.
Mean induced sputum protein concentrations averaged over the 3 patient visits:
| Sputum Protein | COPD non-smokers | COPD smokers | Control non-smokers | Control smokers | P value COPD vs controls |
|---|---|---|---|---|---|
| Eotaxin2 (pg/mg)# | 1244.2 (1638.6) | 808.9 (349.6) | 297.8 (279.1) | 1159.5 (1477.7) | 0.16 |
| HGF (ng/mg)# | 19.2 (15.4) | 18.0 (17.0) | 7.6 (5.9) | 23.3 (20.9) | 0.61 |
| IP-10 (pg/mg)# | 1015.3 (814.8) | 605.8 (393.0) | 395.7 (272.9) | 480.6 (988.8) | 0.94 |
| KGF (pg/mg)# | 771.5 (747.7) | 881.4 (851.5) | 549.9 (516.4) | 1490.1 (1256.2) | 0.67 |
| MMP-13 (ng/mg)# | 29.5 (25.8) | 30.9 (23.8) | 15.1 (11.7) | 45.8 (38.7) | 0.99 |
| TNF-RI (pg/mg)# | 2459.5 (1548.9) | 2635.7 (2280.7) | 1390.2 (1241.6) | 3671.3 (2806.4) | 0.77 |
| GROα (ng/mg)# | 54.9 (63.8) | 23.3 (18.3) | 7.6 (9.6) | 15.7 (14.6) | 0.007 |
| TIMP2 (ng/mg)# | 21.1 (9.2) | 18.6 (17.0) | 5.8 (5.9) | 11.8 (14.4) | 0.09 |
| TNF-RII (pg/mg)# | 169.1 (113.9) | 255.9 (367.0) | 46.7 (42.1) | 130.4 (129.8) | 0.04 |
| α2 macroglobulin (mg/mg)* | 0.008 (0.006) | 0.004 (0.002) | 0.005 (0.003) | 0.06 (0.07) | 0.09 |
| ENA-78 (ng/mg)* | 3.7 (4.1) | 1.9 (1.7) | 1.4 (1.6) | 1.2 (0.8) | 0.36 |
| IL-13 (pg/mg)* | 21.3 (31.2) | 9.3 (7.1) | 8.6 (1.9) | 5.7 (1.7) | 0.92 |
| IL-15 (ng/mg)* | 0.17 (0.32) | 0.11 (0.10) | 0.08 (0.03) | 0.04 (0.02) | 0.47 |
| IL-18 (pg/mg)* | 160.4 (189.8) | 118.9 (74.4) | 58.4 (39.1) | 100.8 (53.2) | 0.32 |
| IL-1β (pg/mg)* | 142.6 (170.7) | 404.3 (701.8) | 22.8 (12.3) | 53.2 (23.1) | 0.06 |
| IL-1RA (ng/mg)* | 87.3 (53.1) | 107.6 (87.4) | 60.7 (36.1) | 130.0 (72.1) | 0.64 |
| IL-6 (pg/mg)* | 156.5 (182.3) | 214.4 (194.7) | 13.3 (7.9) | 100.8 (88.4) | 0.00005 |
| IL-8 (ng/mg)* | 13.6 (9.0) | 32.7 (35.2) | 3.5 (3.0) | 9.0 (8.8) | 0.005 |
| MCP-1 (pg/mg)* | 845.2 (1285.2) | 692.2 (957.0) | 71.3 (54.6) | 237.2 (318.6) | 0.01 |
| MMP-2 (ng/mg)* | 55.3 (37.9) | 41.3 (20.4) | 60.1 (71.6) | 42.3 (20.5) | 0.06 |
| TIMP-1 (ng/mg)* | 206.6 (71.5) | 193.4 (68.9) | 75.5 (70.5) | 112.3 (83.5) | 0.004 |
| VEGF(pg/mg)* | 2653.0 (659.6) | 2845.7 (1013.8) | 1200.4 (706.5) | 1733.6 (819.8) | 0.006 |
Values in brackets are the standard deviations of the mean.
P values were assessed using linear mixed effects models adjusted for age and gender (see methods section for details).
All individual sputum protein values are corrected for total sputum protein concentration.
# = Measured using SearchLight multiplex sandwich ELISA
* = Measured using Luminex bead- based suspension array assays
HGF = hepatocyte growth factor, IP-10 = interferon-inducible protein 10, KGF = keratinocyte growth factor, MMP-13 = matrix metalloproteinase 13, TNF-R1 = tumour necrosis factor receptor I, GROα = growth-related oncogene alpha, TIMP-2 = tissue inhibitors of metalloproteinases 2, TNF-RII = tumour necrosis factor receptor 2, ENA-78 = epithelial neutrophil-activating peptide 78, IL = interleukin, IL-1RA = interleukin 1 receptor antagonist, MCP-1 = monocyte chemotactic protein 1, MMP-2 = matrix metalloproteinase 2, TIMP-1 = tissue inhibitors of metalloproteinases 1, VEGF = vascular endothelial growth factor.