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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Gene. 2010 Mar 23;459(1-2):11–23. doi: 10.1016/j.gene.2010.03.006

Fig. 5.

Fig. 5

In vivo association between NF-Y and human AKR1C1 gene promoter. (A) Schematic representation of the nuclear factor-Y subunits A, B and C association with the CCAAT pentanucleotide sequence in the AKR1C1 gene proximal promoter region. (B) and (C) Chromatin immunoprecipitation assay for detection of the in vivo association between NF-Y and the human AKR1C1 gene promoter. (B) PCR product amplified from primers utilized from an unrelated part of the AKR1C1 gene which is void of the CCAAT pentanucleotide binding site for NF-Y transcription factor binding. (C) The immunoprecipitated DNA obtained from ChIP is amplified utilizing PCR primers specific for a region containing the NF-Y transcription factor binding site in the AKR1C1 gene promoter.