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. 2010 Mar 31;30(13):4757–4766. doi: 10.1523/JNEUROSCI.6108-09.2010

Figure 6.

Figure 6.

The mobility of GFP-SAP102 is rapidly regulated by synaptic activity. A, The images represent FRAP of a neuron expressing GFP-SAP102 with blockade of NMDA and AMPA receptors. Neurons were incubated with 100 μm DL-APV and 20 μm DNQX 1 h before experiments and during experiments. Scale bar, 2 μm. B, C, FRAP of GFP-SAP102 was reduced by blocking both NMDA and AMPA receptor activity. The immobile and slow mobile fractions of GFP-SAP102 decreased after APV and DNQX treatment. There was no change in rapid mobile fractions after APV and DNQX treatment. n = 4 for each FRAP curve, *p < 0.05, Ctrl, Control (black); A+D, APV+DNQX (gray). D, The images represent FRAP of a neuron expressing GFP-PSD-95 with blockade of NMDA and AMPA receptors. Scale bar, 1 μm. E, F, FRAP of GFP-PSD-95 was not affected by APV and DNQX treatment. There was no change in immobile, slow mobile, and rapid mobile fractions after APV and DNQX treatment. n = 4 for each FRAP curve.