Table 2.

Effect of IFG tartrate on L444P GCase activity in lysates from Gaucher patient-derived fibroblasts after IFG removal.

Cell ID	GCase Activity (% increase)
	IFG tartrate (μM)
	6	20	60	6	20	60	6	20	60
	Glycoprotein-enrichment			Immunocapture				IFG Washout
GM07968	64±13	88±19	115±33	33±16	55±17	115±46	40±12	55±13	74±5
GM00877	99±21	113±39	100±20	27±6	41±10	39±8	33±3	51±7	61±12
GM10915	25±5	55±1	100±7	55±12	84±19	73±19	25±3	45±2	55±2
GM08760	ND	ND	ND	38±7	62±6	72±7	ND	ND	ND

GCase activity was measured in Gaucher patient-derived fibroblasts homozygous for L444P GCase after five-day incubation with the indicated concentrations of IFG tartrate. Prior to assay, glycoproteins or GCase were enriched from cell lysates using either Con A- or immunocapture, respectively, or cells were incubated for 24 hours in media only (IFG washout), as described in the ‘Materials and Methods’. The data presented are the mean±SEM from three independent experiments. ND, not determined.