Fig. 3.

(a) In-situ hybridization studies using a full-length Hlx antisense probe (Fig. 3a) and an Hlx sense probe at E11.5 (first panel), E12.5 (second panel), E13.5 (third panel) and E14.5 (fourth panel). In the E11.5 antisense panel: He, heart; hp, hepatic primordium. The developing diaphragm is located between these two structures (37). In the E12.5, E13.5 and E14.5 antisense panels, the arrows indicate the developing diaphragm. Expression of Hlx can be seen in the murine diaphragm and adjacent liver capsule with the antisense probe but not the sense probe, and expression appears strongest at E13.5. Hlx is also strongly expressed in the developing liver. (Fig. 3b) shows a higher magnification view (40×) from the E13.5 antisense section, site indicated by the box in Fig. 3a. Diaphragm staining is indicated by an arrow.