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. 2010 Jun;47(10):1931–1941. doi: 10.1016/j.molimm.2010.02.020

Supplementary Fig. I.

Supplementary Fig. I

Bst NI fingerprint analyses of scFv clones. Bst NI RFLP analysis of scFv clones isolated from a phagemid library panned against TeNT-Hc and verified as target specific by ELISA. The scFv genes were amplified from minipreped phagemid vector by PCR using primers LMB3 and FDSEQ1. The product was digested with Bst NI and then underwent electrophoretic separation on a 4% agarose gel stained with ethidium bromide. Marker is Hyperladder IV (Bioline). Gel A (HcC clones), Gel B (HcJ clones) and Gel C (HcN clones). Clone numbers refer to original screening designation.