Fig. 2.
Anti-tetanus scFv epitope binning analysis. Each of the 14 phage-scFv fusions C1-C5, J1-J5 (excluding J3) and N1-N5 were assayed for binding to TT-Hc systematically at pre-determined fixed dilutions in the absence of soluble scFv and in the presence of each of the 14 scFvs in soluble non-phage-fused form (3–5 μM). Each phage-scFv was assayed against its non-fused form as an intrinsic positive control for competitive measurement. Each condition was measured in triplicate and bars are means with standard error bars. One-way ANOVA was used to determine whether there was significant global variation between phage-binding in the absence of scFvs and in the presence of scFvs for each clone. Significant variation was confirmed in each case (p < 0.01). A post-ANOVA pair-wise multi-comparative test was performed using the Dunnett algorithm which compares mean individual binding measurements with the positive binding control for each clone taking into account global background variation. Significant differences to the no scFv control are summarised in Table 1 and are denoted in this figure with significance of *p < 0.05 or **p < 0.01.