Table 4.
Clone | Model | Chi2 | (M−1 s−1) | SE- (M−1 s−1) | (s−1) | SE- (s−1) | (s−1) | SE- (s−1) | (s−1) | SE- (s−1) | KD (M) |
---|---|---|---|---|---|---|---|---|---|---|---|
C1 | A | 0.463 | 1.61 × 104 | 219 | 1.42 × 10−4 | 3.73 × 10−7 | n/a | n/a | n/a | n/a | 8.82 × 10−9 |
C4 | A | 0.938 | 2.32 × 104 | 103 | 3.11 × 10−4 | 7.28 × 10−7 | n/a | n/a | n/a | n/a | 1.34 × 10−8 |
J2a | A | 0.45 | 1.41 × 104 | 323 | 3.68 × 10−4 | 1.07 × 10−5 | n/a | n/a | n/a | n/a | 2.61 × 10−8 |
J2b | B | 24.5 | 7.9 × 104 | 626 | 2.58 × 10−3 | 1.04 × 10−4 | 3.98 × 10−3 | 1.91 × 10−4 | 1.83 × 10−3 | 4.3 × 10−5 | 1.00 × 10−8 |
J2b | A | 23.3 | 7.67 × 104 | 682 | 7.10 × 10−4 | 2.41 × 10−6 | n/a | n/a | n/a | n/a | 9.25 × 10−9 |
J4 | B | 9.42 | 5.22 × 104 | 403 | 1.65 × 10−2 | 1.28 × 10−4 | 1.94 × 10−3 | 2.11 × 10−5 | 2.48 × 10−3 | 2.28 × 10−5 | 1.77 × 10−7 |
N4 | B | 0.25 | 1.61 × 104 | 312 | 1.81 × 10−2 | 1.67 × 10−4 | 2.24 × 10−3 | 1.53 × 10−5 | 3.8 × 10−4 | 1.43 × 10−5 | 1.63 × 10−7 |
N5a | A | 0.09 | 1.34 × 104 | 325 | 7.43 × 10−4 | 5.2 × 10−6 | n/a | n/a | n/a | n/a | 5.55 × 10−8 |
N5b | A | 6.59 | 1.98 × 104 | 6.25 | 2.66 × 10−4 | 4.99 × 10−7 | n/a | n/a | n/a | n/a | 1.34 × 10−8 |
Model A: Langmuir 1:1 binding. Model B: two-state reaction with conformational change.
Low Rmax surface.
Moderate Rmax surface.