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Schematic representation of FLIPR assay to detect new AMPA receptor positive modulators: •Intracellular calcium change can be detected by calcium-sensitive dyes using the fluorescent imaging plate reader (FLIPR). hGluA2(flip) Q/R edited to allow Ca2+ permeability •HEK293 cells expressing hGluA2 receptor are loaded with Fluo4-AM •AM-ester is cleaved by intracellular esterases •Fluo4 binds to intracellular Ca2+ and increases fluorescence.
