Figure 6.

APoC preserves ΔΨ_m in isolated myocytes throughout reoxygenation. (A) Representative recording of ΔΨ_m changes during H/R. Hypoxia caused a significant decrease in the standard deviation of TMRE fluorescence indicating mitochondrial depolarization whereas reoxygenation caused an increase in the standard deviation indicating recovery of ΔΨ_m. Treatment of cardiomyocytes with isoflurane at the beginning of reoxygenation preserved ΔΨ_m as compared with control cells. (B) Summary data for control and APoC groups at 10 min of reoxygenation. (C) Effect of isoflurane on mitochondrial flavoprotein fluorescence, and summary data (inset). (D) Representative trace of ΔΨ_m in isolated mitochondria. Isoflurane induced slight depolarization of ΔΨ_m. Data are means ± standard deviation; n= 15; n= 6 in flavoprotein and isolated mitochondria experiments. *P < 0.05 versus control. APoC, isoflurane postconditioning, TMRE, tetramethylrhodamine ethyl ester. FCCP, carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone.