Figure 3.

Effect of β₂-adrenoceptor agonists and cAMP-elevating agents on NF-κB-dependent transcription. BEAS-2B 3κBu luciferase reporter cells were either left untreated or were incubated with: (A) salbutamol (1 µM), salmeterol (0.1 µM) or formoterol (0.01 µM), or (B) forskolin (10 µM) or PGE₂ (1 µM) for 1 h prior to either being left not stimulated (NS) or stimulated with IL-1β (1 ng·mL⁻¹), or IL-1β (1 ng·mL⁻¹) and histamine (100 µM) (I + H). Cells were harvested after 6 h for luciferase activity determination. Data (n= 5–6), expressed as fold activation, are plotted as means ± SEM. *P < 0.05, ***P < 0.001 versus IL-1β, ##P < 0.01 versus I + H.