Figure 4.

Effect of salmeterol on AP-1- and CRE-dependent transcription. BEAS-2B cells (A) transiently transfected with pGL3.neo.TATA.3AP-1 or (B) stably transfected with the CRE luciferase reporter were either left untreated or were incubated with salmeterol (0.1 µM) for 1 h prior to either being left not stimulated (NS) or stimulated with histamine (100 µM) (His), IL-1β (1 ng·mL⁻¹) or IL-1β (1 ng·mL⁻¹) plus histamine (100 µM) (I + H). Cells were harvested after 6 h for luciferase activity determination. Data (n= 3–5), expressed as fold activation, are plotted as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 versus NS.