Figure 5.

Effect of salmeterol concentration on IL-1β plus histamine-induced IL-6 and IL-8 release. BEAS-2B cells were either left untreated and either left not stimulated (NS) or stimulated with IL-1β (1 ng·mL⁻¹), or IL-1β (1 ng·mL⁻¹) plus histamine (100 µM), or were incubated with various concentrations of salmeterol for 1 h prior to being stimulated with IL-1β (1 ng·mL⁻¹) and histamine (100 µM) (I + H). Cells were harvested after 6 h, and the supernatants were analysed by elisa for IL-6 and IL-8 release. Data (n= 6), expressed as pg·mL⁻¹, are plotted as means ± SEM. *P < 0.05, **P < 0.01 versus I + H.