FIGURE 4.
Confocal z-stack series through a GAP-43/ZnT3 double-immunostained hippocampal section demonstrates that GAP-43+ structures are distinct from ZnT3+ puncta. Three separate 40X optical sections, separated by 0.5 µm, of double-immunostained tissue from the dorsal hippocampal stratum lucidum of a G-Phos mouse are presented in (a). Note the lack of colocalized puncta, highlighted by the zoomed in areas in (b). Scale bar in (a) = 20 µm; scale bar in (b) = 5 µm. [Color figure can be viewed in the online issue, which is available at www.interscience.wiley.com.]