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. Author manuscript; available in PMC: 2011 May 7.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Apr 9;395(3):395–400. doi: 10.1016/j.bbrc.2010.04.028

Fig. 3. Pentose phosphate pathway is not involved in PP2A-independent activation of ChREBP.

Fig. 3

(A) pGAMPAC-ChREBP, pG5-luc, pRL-TK and indicated amount of pCMX-G6PD were infected into 832/13 cells. The total amount of transfected DNA was held the same by adding appropriate amount of vector DNA. Transfected cells were subsequently cultured under 2.5 mM low glucose over night, and then treated with either low (2.5 mM) or high (27.5 mM) glucose for 6 hrs before luciferase assay. * p< 0.05; ** p< 0.005 vs vector only transfected at 27.5 mM glucose. (B) Real-time PCR measurement of mRNA content of G6PD in 832/13 cells infected with lentivirus expressing shRNA against G6PD (knockdown) or its scrambled counterpart (ctrl). Data are normalized to mRNA level of the Eukaryotic Elongation factor 1 gamma (EEF1g). * < 0.05 vs ctrl. (C) Luciferase assay: pGAMPAC-ChREBP, pG5-luc and pRL-TK were transfected into 832/13 cells infected with lentivirus expressing either shRNA against G6PD (knockdown) or scrambled shRNA (ctrl). The transfected cells were then cultured in low glucose overnight, and treated with either 2.5 mM or 27.5 mM glucose for 6 hrs before luciferase assay. * < 0.05 vs ctrl at 27.5 mM glucose.