Skip to main content
. Author manuscript; available in PMC: 2011 May 7.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Apr 9;395(3):395–400. doi: 10.1016/j.bbrc.2010.04.028

Fig. 4. Glycolytic pathway is not involved in PP2A-independent activation of ChREBP.

Fig. 4

(A) pGAMPAC-ChREBP, pG5-luc, and pRL-TK were transfected into 832/13 cells, which were subsequently cultured under low glucose overnight, and then treated with indicated concentrations of 2-DG and/or D-mannoheptulose for 6 hrs before luciferase assay. DG15, 15 mM 2-DG; DG30, 30 mM 2-DG; M10, 10 mM D-mannoheptulose:* p < 0.05; ** p < 0.005 vs ctrl; # p< 0.05 vs DG30. (B) pGAMPAC-ChREBP, pG5-luc, and pRL-TK were cotransfected with control vector, pCMX-PFK1 and/or pIRESpuro-iPFK2 (0.15 μg each) into 832/13 cells. The total amount of transfected DNA was held the same by adding appropriate amount of vector DNA. Transfected cells were subsequently cultured under low glucose overnight, and then treated with either 2.5 mM or 27.5 mM glucose for 6 hrs before luciferase assay. ** p < 0.005 vs ctrl at high glucose.