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Protease-activated receptor-1 (PAR1) signaling through Gαi/o is critical for epidermal growth factor (EGF) receptor (EGFR) and ErbB2 transactivation. (a and b) Serum-starved MDA-MB-231 cells were pretreated with 100 ng/ml pertussis toxin for 18 h at 37 °C or left untreated and then incubated in the absence or presence of 10 nM thrombin for 5 or 30 min at 37 °C. Cells were processed and EGFR and ErbB2 tyrosine phosphorylation was measured as described in Figure 1.
