A, DU-145 cells were infected with Ad.mda-7 or Ad.vec for different times and LC3 expression was analyzed by Western blotting. B, LC3 expression was examined after 24 h treatment with Ad.mda-7 or Ad.vec (100 pfu/cell) in the presence of rapamycin (100 nM), or bafilomycin A1 (100 nM). C, Cells were transfected with the indicated siRNAs and LC3-GFP followed by infection with 100 pfu/cell Ad.mda-7 or Ad.vec and cytoplasmic aggregation of LC3-GFP was determined. A minimum of 100 GFP-LC3–transfected cells were counted. *, P < 0.05; **, P < 0.001, compared with si control. D, LC3-II expression 24 h after administration of the indicated siRNAs and Ad.mda-7 infection (100 pfu/cell) by immunoblot. Densitometry was performed on the original blots, and the ratio of LC3-II/actin in control cells was 1.