Skip to main content
. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Cancer Res. 2010 Apr 20;70(9):3667–3676. doi: 10.1158/0008-5472.CAN-09-3647

Fig. 5. Role of Beclin-1 and ATG5 in Ad.mda-7-induced apoptosis.

Fig. 5

DU-145 cells were transfected with the indicated siRNAs and cell viability was determined by MTT assay (A), Caspase-Glo(R) 3/7 assay (B) for caspase-3 expression and colony formation assays (C) 48 h after infection with Ad.mda-7 (10, 50 or 100 pfu/cell) or Ad.vec (100 pfu/cell). C, Colony formation assays in monolayer culture. Colonies were fixed, stained and counted (> 50 cells) 2 weeks after plating. D, Expression profile of Beclin-1 and ATG5 at the mRNA level 48 h after Ad.mda-7-infection (50 or 100 pfu/cell) of DU-145 cells (*, P < 0.05; **, P < 0.001, compared with control).