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Parameter estimation through curve fitting.
| Curve | Parameters | |
|---|---|---|
| Γm (pmoles/cm2) | Kd (nmoles/L) | |
| Particle dilution | 4 ± 0.13 | 0.28 ± 0.004 |
| Analyte dilution (one site model in the ambient analyte region) |
0.36 ± 0.03 | |
| Analyte dilution (two site model in the ambient analyte region) |
Kd1 0.11±0.02 Kd2 1.57±0.29 |
|
| Analyte dilution (non ambient analyte region) |
3.8 ± 0.09 | 0.27 ± 0.06 |
| BD Quantibrite PE Beads | 2.54 | |
