Figure 1. Measurement of H2Sreleased from oral bacteria.
Fourteen bacterial strains that belong to different pathogenesis clustering complexes were cultured in anaerobic conditions until they reached the late log growth phase. The concentration of all strains was adjusted to 0.9 as measured by OD 590 nm. The concentrations of H2S and H2 were measured in the bacterial culture broth using a needle-type H2S or H2 sensor, as described in the Materials & Methods section. Data are shown as the mean ± SD of three different cultures.