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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Nov 22;391(1):645–650. doi: 10.1016/j.bbrc.2009.11.113

Figure 4. Effects of RGP and KGP deletion mutant strains of Pg on IL-8 production in the culture of gingival epithelial cells.

Figure 4

(A) The culture broth of wild-type Pg 33277 or Pg KDP137 (RGP-KGP-HagA triple deficient mutant strain) growing in log growth phase was tested for H2S concentration using a needle-type H2S sensor. Data represent the average ± SD of three cultures. (B) OBA9 cells were cultured in the lower chamber, and Pg KDP137 was applied to the upper chamber of the transwell co-culture system in the presence of PMA (1mmol/L) with or without Morpholine-resin. After co-culture for 48 hr, the concentrations of IL-8 in the culture supernatant were measured by ELISA. Data are the mean ± SD of three cultures. *p < 0.05, **p < 0.01: Values differ significantly (t-test).