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Comparison of energy transfer efficiency (E%) using various methods for selected FRET standards and cells. sFRET: contaminated with acceptor bleedthrough, ASBT. (number of cells, 12).
| FRET Pair | sFRET (%) | psFRET (%) | PFRET (%) | sRET (%) | FLIM-FRET (%) |
|---|---|---|---|---|---|
| CTV | 18 (±6) | 7 (±4) | 8 (±2) | 7 (±5) | 8 (±2) |
| CVC | 52 (±5) | 43 (±3) | 44 (±4) | 40 (±5) | 42 (±3) |
| CFP + YFP-C/EBPα | 41 (±4) | 26 (±3) | 23 (±5) | — | 26 (±3) |
| CFP – C/EBPα + YFP-Pit-1 | 31 (±4) | 14 (±3) | 13 (±3) | — | 16 (±4) |
