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. Author manuscript; available in PMC: 2010 Jun 15.
Published in final edited form as: J Immunol. 2009 Jun 15;182(12):8110–8117. doi: 10.4049/jimmunol.0900300

Figure 1.

Figure 1

Generation and characterization of recombinant neutralizing anti-IgE single chain antibody construct.

(A) The gene construct encoding recombinant R1E4 (anti-IgE) showing intron/exon structure and selected features. Introns are depicted as thin lines. R1E4 scFv express under the control of human ubiquitin promoter. The R1E4 scFv was fused to hinge and Fc encoding exons of mouse IgG1 H-chain. For membrane bound anti-IgE expression H-2Kb major histocompatibility complex class I transmembrane coding sequences were included downstream.

(B) Control or recombinant plasmid expressing the membrane form of single chain R1E4 were transiently co-transfected with EGFP plasmid into 293 cells and the ability of the cells to bind to IgE was analyzed by flow cytometry.