Figure 5.
Systemic anaphylaxis reactions in rR1E4-treated mice.
(A) Untreated BALB/c (WT) mice or pR1E4-treated (Sec) mice were challenged with 100μg activating anti-IgE mAb EM95 and rectal temperatures were measured over 60 minutes.
(B) Mice received 0.5 mg Fc blocking mAb 2.4G2 twenty-four hours before challenge with 100μg anti-IgE EM95. Untreated BALB/c (WT+Fc), pR1E4-treated (Sec+Fc).
(C) FcεRIα-/- mice received 10μg TNP-BSA and 100μg anti-TNP IgE (IgELa) one hr before challenge with 50μg rR1E4. Shown are means and standard deviations of results obtained with the following numbers of mice/group. All groups included 3 mice except pR1E4-treated mice in A which included 4 mice.