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. Author manuscript; available in PMC: 2010 Nov 20.
Published in final edited form as: Nature. 2010 May 20;465(7296):359–362. doi: 10.1038/nature09022

Figure 3. Calcium-dependent microneme secretion requires TgCDPK1.

Figure 3

a, Western blot analysis of microneme protein MIC2 secretion following induction by ethanol for 15 min. GRA1 shows constitutive secretion of dense granules. Student’s t test; **P < 0.005, mean ± s.e.m., N = 3 experiments. b, Ionophore-induced permeabilization detected by vacuolar DsRed leakage monitored by fluorescence video-microscopy of strains 90h + ATc . Time stamp (min:sec) after calcium ionophore addition. CytochalasinD added to prevent eggress. c-d, Quantification of maximal rate and timing of florescence loss from rupturing vacuoles. Mann-Whitney test; ***P < 0.0005, **P < 0.005, mean, N = 3 experiments.