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. Author manuscript; available in PMC: 2010 Nov 20.
Published in final edited form as: Nature. 2010 May 20;465(7296):359–362. doi: 10.1038/nature09022

Figure 4. PP1 derivatives specifically inhibit TgCDPK1 and block micronem-mediated functions.

Figure 4

a, Alignment of the kinase sub-domain V highlighting the gatekeeper residue. b, Structures of 3-MB-PP1 and 3-BrB-PP1. c, Effect of 5μM 3-MB-PP1 on host cell invasion. Student’s t test; *P < 0.05, mean ± s.e.m., N = 3 experiments. d, Effect of 5μM 3-MB-PP1 on MIC2 secretion. Student’s t test; *P < 0.05, mean ± s.e.m., N = 3 experiments. e-f, Effect of PP1 derivatives on host lysis by T. gondii +/− 3-MB-PP1 (e) and 3-Br- PP1 (f). Mean ± s.e.m., N = 3 experiments.