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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: J Immunol. 2010 May 5;184(11):6350–6358. doi: 10.4049/jimmunol.0902673

FIGURE 4.

FIGURE 4

MSU crystals modulate eosinophil viability. (A) Eosinophils were incubated with medium alone or serial dilutions of MSU crystal suspensions for 3 h or 24 h at 37 °C. (B) Eosinophils were incubated with medium alone or serial dilutions of soluble uric acid (UA) for 3 h or 24 h at 37 °C. Cell apoptosis and death were examined by double staining with FITC-conjugated annexin V and PI and flow cytometry analysis. The percentages of apoptotic cells (annexin V-positive and PI-negative) and dead cells (annexin V-positive and PI-positive) were determined. The proportion of cells with annexin V-negative and PI-positive was less than 0.5%. Results show the mean±SEM from five (A) or three (B) different eosinophil preparations. Downward error bars and upward error bars indicate SEM for apoptotic cells and dead cells, respectively. * and #; significant differences compared with percent apoptotic cells and dead cells in medium alone, respectively (p<0.01).