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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: J Immunol. 2010 May 5;184(11):6350–6358. doi: 10.4049/jimmunol.0902673

FIGURE 8.

FIGURE 8

Blockade of P2 receptors with oATP inhibits EDN release and decreases cell death induced by MSU crystals. Eosinophils were preincubated with serial dilutions of oATP for 15 min and incubated with or without 3 mg/ml MSU crystal suspensions for 3 h at 37 °C. (A) EDN released into supernatants was measured by ELISA. (B) Eosinophil viability was examined by staining cells with PI and fluorescein diacetate and by analyzing at least 200 cells with an epifluorescent microscope. The percentages of dead cells (PI-positive and flurorescein diacetate-negative) were determined. Results show the mean±SEM from five different eosinophil preparations. *; significant difference compared with no MSU (p<0.05).