Table 1.
List of DIGE-identified proteins with a changed abundance in 9-month old dystrophic MDX heart muscle.
| Spot No. | Protein name | Protein ID | Peptides matched | Coverage (%) | Molecular mass (kDa) | Isoelectric point (pI) | Mascot score | Fold change |
|---|---|---|---|---|---|---|---|---|
| 1 | Nucleoside diphosphate kinase B | NDKB_MOUSE | 4 | 34 | 17.5 | 6.9 | 69 | 4.4 |
| 2 | Lamin-A/C | LMNA_MOUSE | 4 | 6 | 74.5 | 6.5 | 111 | 1.9 |
| 3 | Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial | ETFD_MOUSE | 8 | 15 | 68.9 | 7.3 | 45 | 1.8 |
| 4 | Myozenin-2 | MYOZ2_MOUSE | 4 | 20 | 29.8 | 8.5 | 145 | 0.67 |
| 5 | Electron transfer flavoprotein subunit alpha, mitochondrial | ETFA_MOUSE | 2 | 12 | 35.3 | 8.6 | 89 | 0.67 |
| 6 | Prohibitin | PHB_MOUSE | 8 | 41 | 29.9 | 5.6 | 290 | 0.67 |
| 7 | Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial | ODO2_MOUSE | 3 | 6 | 49.3 | 9.1 | 86 | 0.63 |
| 8 | Isovaleryl-CoA dehydrogenase, mitochondrial | IVD_MOUSE | 7 | 23 | 46.7 | 8.5 | 89 | 0.59 |
| 9 | Serum albumin | ALBU_MOUSE | 3 | 5 | 70.7 | 5.8 | 82 | 0.59 |
| 10 | Cytochrome b-c1 complex subunit Rieske, mitochondrial | UCRI_MOUSE | 4 | 13 | 29.6 | 8.9 | 84 | 0.56 |
| 11 | Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial | ODP2_MOUSE | 10 | 20 | 68.5 | 8.8 | 117 | 0.53 |
| 12 | Vimentin | VIME_MOUSE | 11 | 20 | 53.7 | 5.1 | 118 | 0.53 |
| 13 | Beta-enolase | ENOB_MOUSE | 8 | 19 | 47.3 | 6.7 | 111 | 0.50 |
| 14 | Pyruvate dehydrogenase E1 component subunit beta, mitochondrial | ODPB_MOUSE | 8 | 31 | 39.3 | 6.4 | 279 | 0.50 |
| 15 | Pyruvate dehydrogenase E1 component subunit beta, mitochondrial | ODPB_MOUSE | 3 | 19 | 39.3 | 6.4 | 134 | 0.50 |
| 16 | Creatine kinase M-type | KCRM_MOUSE | 2 | 4 | 43.3 | 6.6 | 47 | 0.50 |
| 17 | Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial | ECH1_MOUSE | 4 | 24 | 36.4 | 7.6 | 201 | 0.50 |
| 18 | Heat shock protein beta-1 Hsp27 | HSPB1_MOUSE | 2 | 8 | 23.1 | 6.1 | 50 | 0.50 |
| 19 | Isovaleryl-CoA dehydrogenase, mitochondrial | IVD_MOUSE | 7 | 17 | 46.7 | 8.5 | 98 | 0.50 |
| 20 | Pyruvate dehydrogenase protein X component, mitochondrial | ODPX_MOUSE | 5 | 11 | 54.3 | 7.6 | 63 | 0.48 |
| 21 | Actin, alpha cardiac muscle 1 | ACTC_MOUSE | 6 | 19 | 42.3 | 5.2 | 104 | 0.48 |
| 22 | Cytochrome c oxidase subunit 5B, mitochondrial | COX5A_MOUSE | 2 | 14 | 14.1 | 8.7 | 52 | 0.48 |
| 23 | Vimentin | VIME_MOUSE | 13 | 27 | 53.7 | 5.1 | 122 | 0.48 |
| 24 | Transitional endoplasmic reticulum ATPase (valosin-containing protein VCP) | TERA_MOUSE | 5 | 10 | 89.9 | 5.1 | 171 | 0.46 |
| 25 | Pyruvate dehydrogenase E1 component subunit beta, mitochondrial | ODPB_MOUSE | 4 | 14 | 39.3 | 6.4 | 100 | 0.44 |
| 26 | Myosin regulatory light chain 2, ventricular/cardiac muscle isoform | MLRV_MOUSE | 9 | 52 | 18.9 | 4.9 | 209 | 0.44 |
| 27 | Fatty acid-binding protein FABP-3, heart | FABPH_MOUSE | 5 | 37 | 14.8 | 6.1 | 149 | 0.44 |
| 28 | Enolase 1, alpha | ENOA_MOUSE | 2 | 3 | 47.5 | 6.4 | 43 | 0.44 |
| 29 | DJ-1 protein | PARK7_MOUSE | 4 | 26 | 20.2 | 6.3 | 90 | 0.44 |
| 30 | Cytochrome b-c1 complex | QCR1_MOUSE | 6 | 17 | 53.4 | 5.8 | 83 | 0.44 |
| 31 | 60 kDa heat shock protein, Hsp60, mitochondrial | CH60_MOUSE | 2 | 6 | 61.1 | 5.9 | 63 | 0.42 |
| 32 | Beta-enolase | ENOB_MOUSE | 3 | 7 | 47.3 | 6.7 | 92 | 0.42 |
| 33 | Fatty acid binding protein, FABP-3, heart | FABPH_MOUSE | 7 | 52 | 14.8 | 6.1 | 162 | 0.42 |
| 34 | Pyruvate dehydrogenase protein X component, mitochondrial | ODPX_MOUSE | 2 | 4 | 54.3 | 7.6 | 66 | 0.42 |
| 35 | Tropomyosin alpha-1 chain | TPM1_MOUSE | 15 | 43 | 32.7 | 4.7 | 302 | 0.40 |
| 36 | Electron transfer flavoprotein subunit alpha, mitochondrial | ETFA_MOUSE | 8 | 33 | 35.3 | 8.6 | 235 | 0.40 |
| 37 | 60 kDa heat shock protein, Hsp60, mitochondrial | CH60_MOUSE | 12 | 32 | 61.1 | 5.9 | 346 | 0.40 |
| 38 | Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial | IDH3A_MOUSE | 9 | 29 | 40.1 | 6.3 | 164 | 0.40 |
| 39 | Actin, alpha cardiac muscle 1 | ACTC_MOUSE | 12 | 31 | 42.3 | 5.2 | 317 | 0.39 |
| 40 | NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial | NDUV2_MOUSE | 6 | 29 | 27.6 | 7.0 | 370 | 0.39 |
| 41 | Voltage-dependent anion-selective channel protein VDAC1 | VDAC1_MOUSE | 10 | 29 | 32.5 | 8.6 | 292 | 0.39 |
| 42 | Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial | IDH3A_MOUSE | 13 | 39 | 40.1 | 6.3 | 376 | 0.39 |
| 43 | Desmin | DESM_MOUSE | 30 | 50 | 53.5 | 5.2 | 1184 | 0.39 |
| 44 | 60 kDa heat shock protein, Hsp60, mitochondrial | CH60_MOUSE | 25 | 38 | 61.1 | 5.9 | 842 | 0.37 |
| 45 | Actin, alpha cardiac muscle 1 | ACTC_MOUSE | 10 | 26 | 42.3 | 5.2 | 276 | 0.36 |
| 46 | Actin, alpha cardiac muscle 1 | ACTC_MOUSE | 4 | 14 | 42.3 | 5.2 | 88 | 0.36 |
| 47 | Desmin | DESM_MOUSE | 15 | 37 | 53.5 | 5.2 | 349 | 0.36 |
| 48 | Myosin regulatory light chain 2, atrial isoform | MLRA_MOUSE | 9 | 57 | 19.6 | 4.8 | 299 | 0.36 |
| 49 | Fatty acid-binding protein, FABP-3, heart | FABPH_MOUSE | 12 | 61 | 14.8 | 6.1 | 425 | 0.35 |
| 50 | ATP synthase subunit beta, mitochondrial | ATPB_MOUSE | 18 | 40 | 56.3 | 5.2 | 1093 | 0.35 |
| 51 | Cytochrome c oxidase subunit 5A | COX5A_MOUSE | 7 | 28 | 16.3 | 6.1 | 286 | 0.35 |
| 52 | Desmin | DESM_MOUSE | 26 | 62 | 53.5 | 5.2 | 1657 | 0.35 |
| 53 | Myosin light chain 3 | MYL3_MOUSE | 6 | 41 | 22.5 | 5.0 | 90 | 0.35 |
| 54 | Desmin | DESM_MOUSE | 12 | 30 | 53.5 | 5.2 | 431 | 0.33 |
| 55 | ATP synthase subunit d, mitochondrial | ATP5H_MOUSE | 3 | 21 | 18.8 | 5.5 | 48 | 0.33 |
| 56 | Actin, alpha cardiac muscle 1 | ACTC_MOUSE | 16 | 40 | 42.3 | 5.2 | 598 | 0.32 |
| 57 | Cytochrome b-c1 complex | QCR1_MOUSE | 12 | 27 | 53.4 | 5.8 | 330 | 0.32 |
| 58 | Desmin | DESM_MOUSE | 36 | 68 | 53.5 | 5.2 | 1289 | 0.32 |
| 59 | Tropomyosin alpha-1 chain | TPM1_MOUSE | 21 | 46 | 32.7 | 4.7 | 605 | 0.32 |
| 60 | Actin, alpha cardiac muscle 1 | ACTC_MOUSE | 18 | 59 | 42.3 | 5.2 | 450 | 0.31 |
| 61 | Myosin light chain 3 | MYL3_MOUSE | 11 | 63 | 22.5 | 5.0 | 231 | 0.30 |
| 62 | Myosin regulatory light chain 2, ventricular/cardiac muscle isoform | MLRV_MOUSE | 13 | 73 | 18.9 | 4.9 | 118 | 0.29 |
| 63 | Cytochrome b-c1 complex | QCR1_MOUSE | 12 | 31 | 53.4 | 5.8 | 342 | 0.29 |
| 64 | Actin, alpha cardiac muscle 1 | ACTC_MOUSE | 7 | 36 | 42.3 | 5.2 | 160 | 0.29 |
| 65 | Myosin regulatory light chain 2, ventricular/cardiac muscle isoform | MLRV_MOUSE | 7 | 46 | 18.9 | 4.9 | 354 | 0.29 |
| 66 | Actin, alpha cardiac muscle 1 | ACTC_MOUSE | 4 | 24 | 42.3 | 5.2 | 91 | 0.28 |
| 67 | Actin, alpha cardiac muscle 1 | ACTC_MOUSE | 17 | 62 | 42.3 | 5.2 | 294 | 0.27 |
| 68 | Adenylate kinase isoenzyme 1 | KAD1_MOUSE | 5 | 31 | 21.6 | 5.7 | 174 | 0.26 |
| 69 | ATP synthase subunit beta, mitochondrial | ATPB_MOUSE | 4 | 21 | 56.3 | 5.2 | 92 | 0.26 |
| 70 | Myosin regulatory light chain 2 | MLRA_MOUSE | 6 | 47 | 19.6 | 4.8 | 168 | 0.25 |
| 71 | Actin, alpha cardiac muscle 1 | ACTC_MOUSE | 18 | 61 | 42.3 | 5.2 | 302 | 0.25 |
| 72 | ATP synthase subunit d, mitochondrial | ATP5H_MOUSE | 5 | 36 | 18.8 | 5.5 | 69 | 0.25 |
| 73 | ATP synthase subunit beta, mitochondrial | ATPB_MOUSE | 18 | 51 | 56.3 | 5.2 | 640 | 0.25 |
| 74 | ATP synthase subunit beta, mitochondrial | ATPB_MOUSE | 25 | 71 | 56.3 | 5.2 | 1102 | 0.25 |
| 75 | Actin, alpha cardiac muscle 1 | ACTC_MOUSE | 6 | 16 | 42.3 | 5.2 | 119 | 0.25 |
| 76 | Myosin light chain 3 | MYL3_MOUSE | 6 | 30 | 22.5 | 5.0 | 99 | 0.25 |
| 77 | ATP synthase subunit beta, mitochondrial | ATPB_MOUSE | 24 | 70 | 56.3 | 5.2 | 871 | 0.24 |
| 78 | Myosin light chain 2, ventricular/cardiac | MLRV_MOUSE | 11 | 77 | 18.9 | 4.9 | 206 | 0.24 |
| 79 | Peroxiredoxin-6 | PRDX6_MOUSE | 3 | 19 | 24.9 | 5.7 | 102 | 0.19 |