Figure 2.

Tregs inhibit CTL function in vitro. (a) Activated splenocytes from DC-treated mice were cocultured with CD4⁺CD25⁺Foxp3⁺ cells (purified from tumor-bearing mice) in different ratios. The percentage of intracellular IFN-γ of CD8+ cells was measured using flow cytometry. And (b) the percentage of intracellular IFN-γ and granzyme B expression was measured using FACS. A reduction in the IFN-γ and granzyme B expression was found especially in those fractions where increasing doses of Treg were added to the CD8⁺ T cells.