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. 2010 Feb 5;38(9):e106. doi: 10.1093/nar/gkq044

Figure 2.

Figure 2.

Schematic overview of the efficiency of FLPo-mediated RMCE in FlEx gene trap ES cell clones. Shown are all transfected gene trap clone insertions and the corresponding rounded efficiency of exchange reaction of pEx-FLP-dsRed in three different reading frames (0 upper, +1 middle and +2 lowest values in percent, red values indicating the frame matching the gene trap insertion). For the Tardbp insertion, the exchange vector pEx-FLP-hTDP-43(A315T) was used. All gene trap clones used had insertions in an intron of the respective gene, and the ratio on the right indicates in which intron out of how many total introns the insertion is located. On the left, the size of the genes is indicated in kilobases, and the total size of the genes is not drawn in scale, with omitted DNA indicated. Untranslated and translated exons according to Ensembl. MGI Gene Identifiers and absolute expression levels in ES cells are listed in Supplementary Table S1.