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. 2010 Jan 27;38(9):2839–2850. doi: 10.1093/nar/gkq012

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Cross-talk between PPARα- and SREBP-dependent gene-regulation. (A) Enriched ChIP-chip signals for HMGCS1, HMGCR, LPIN1 and AGPAT9 genes were visualized using Affymetrix integrated genome browser. Coverage of promoter tiling array is indicated in red, repetitive sequences in black and conserved sequences in blue. (B) Gene expression changes after 6 h PPARα agonist treatment of five direct SREBP target genes and possible SREBP target gene AGPAT9. A GW7647-induced PPARα binding region was assigned to each of these genes. Significant differences are indicated with an asterisk (Student’s t-test, p < 0.05). (C) Transcriptional up-regulation of selected SREBP1 target genes involved in lipogenesis after 6 h PPARα agonist treatment represented as a heat map. (D) Enriched DNA binding of PPARα to promoter regions of LPIN1, AGPAT9 and HMGCR after 2 h GW7647 treatment, verified by ChIP-qPCR using primers designed within the binding region found by ChIP-Chip.