Figure 1.

Overexpression of OGG1–GFP accelerates repair of 8-oxoG induced in KBrO₃-treated cells. (A) 8-OxoG were stained with anti-8-oxoG antibody; DNA was stained with PI after RNAse A digestion. Scale bars, 2 µm. (B) 8-OxoG in untreated and KBrO₃-treated cells were quantified as Fpg-sensitive sites by alkaline elution in HeLa and OGG1–GFP cells. (C) Kinetics of repair of 8-oxoG in HeLa and OGG1–GFP cells. After KBrO₃ treatments cells were allowed to recover for the indicated periods of time and analysed by alkaline elution to measure the remaining 8-oxoG lesions.