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. 2010 Jan 21;38(9):3119–3132. doi: 10.1093/nar/gkq002

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Examination of the binding affinity of U/G-pocket mutants by fluorescence spectroscopy. The panel shows the titration curves [fraction of bound RNA; ΔF/F_max versus total Rna15(16-111) concentration] derived from the change in fluorescence intensity for Rna15(16-111) (dark blue) and substitution mutants Y21F, Y21A, Y27F, Y27A, R87A and R87K upon binding to the Tet-UGUUGU ribo-oligonucleotide. The Binding constants in the inset table were determined from these plots by fitting of a hyperbolic binding isotherm to the data.