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Subnuclear localization of the scaRNA, snoR102 and orphan snoRNA, snoR109. In situ hybridizations with antisense probes of (A) snoR102 and (B) snoR109 labelled bright foci on the periphery of the nucleolus (left panel) when compared to nuclear staining with DAPI (right panel). Darker regions in the DAPI stained nuclei which coincide with the labelled sites are indicated by white arrows. No: nucleolus.
