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. 2010 Jan 19;38(9):3106–3118. doi: 10.1093/nar/gkp1216

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Small-angle X-ray diffraction (SAXS) of both apo and Ni-bound HpNikR. (A) Experimental scattering profiles from the apo (blue circles) and holo (green circles) HpNikR showing that both curves are almost equivalent. Dashed line square shows a close-up of the low-angle region of both experimental curves. (B) Experimental scattering profile from the apo-HpNikR (black spheres) compared with the theoretical curves calculated for HpNikR in closed cis- (green), closed trans- (blue) and open (red) conformations. The best fit is obtained with the closed cis-conformation as shown by the chi between experimental and theoretical curves obtained using Crysol.