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. 2010 Feb 4;38(9):2944–2954. doi: 10.1093/nar/gkp1252

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — A non-consensus heptamer found at the SIL locus destabilizes the RAG SE complex. (A) Representative gels for end release assays, depicted in Figure 1B, using substrates with non-consensus heptamers found at the SCL (6c7c) and SIL (4t5c) locus, note that a consensus nonamer is used to examine post-cleavage effects of heptamer mutations. (B) Quantification of SE release from three experiments (*P < 0.02). (C) Quantification of in vitro cleavage by purified RAG proteins, measured as the combined amount of radioactivity from cleaved products divided by the total amount of radioactivity. Examples of gels are shown in Figure 4A in the proteinase K treated lanes. 12SIL: CACtcTG_ACAAAAACC paired with consensus 23RSS. 12SCL: CACAGcc_ACAAAAACC paired with consensus 23RSS. 12/23SIL: CACtcTG_ACAAAAACC at both RSS. 12/23SCL: CACAGcc_ACAAAAACC at both RSS.