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Effect of increasing oligonucleotide 1 concentration on DNMT1 activity. (A) Real-time data averaged from duplicate assays. Assays contained oligonucleotide 1 at concentrations of 30 nM (black line), 60 nM (orange line), 120 nM (green line) and 240 nM (blue line) with associated negative controls (lacking DNMT1) shown as dotted lines. (B) Plot of steady-state rate against concentration of oligonucleotide 1.
