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. 2010 Feb 5;38(9):e107. doi: 10.1093/nar/gkq047

Table 1.

Oligonucleotide 2 fluorescence calibration curve data

Assay volume (µl) Gain Gradient (AU/nM) R2
20 133 183 ± 7.1 0.990
20 165 1347 ± 29 0.997
100 190 962 ± 50 0.982

R2 is a measure of the goodness of fit to a linear function.