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Macrophages express S1P1 and S1P2 receptors. Expression of S1P receptor mRNA in C57BL/6J and S1P2-deficient bone marrow–derived macrophages were analyzed by conventional RT-PCR. There was no detectable expression of S1P3, S1P4, or S1P5 mRNAs. There was no compensatory upregulation of S1P1 in the S1P2-deficient macrophages. β-Actin was used as a control for sample loading. Data represent pooled RNA samples from 3 mice per group.
